Cardiac morphometric alterations in rats with metabolic syndrome
Keywords:
metabolic syndrome, morphometry, biomodels, cardiomegalyAbstract
Introduction: metabolic syndrome is characterized by histological and functional alterations of the heart and the use of morphometric methods is an essential tool in the evaluation of cardiac cellular morphology.Objective: to describe the histomorphometric differences in the cardiac muscle of a model of healthy rats and another group subjected to a state of metabolic syndrome.
Methods: a developmental, correlational, cross-sectional research study using a system of morphometric methods was conducted in the period from October 2015 to September 2019. Histological heart slices corresponding to 16 male Sprague-Dawley rats, randomly distributed in two experimental groups, were analyzed, with three images taken from each processed heart sample. Descriptive statistical techniques were used to characterize the sample by the different variables studied, and the one-factor ANOVA parametric test for analysis of variance was applied for group comparisons.
Results: there was variability in the morphometric parameters studied and the diseased group offered the highest values of cellular area and nuclear area. These findings are consistent with the natural evolution of left ventricular hypertrophy in the course of metabolic syndrome.
Conclusions: muscle fiber area and nuclear area were higher in the induced treated group.
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1. Aguilera Eugia R, Vergara Miranda C, Quezada Donoso R, Sepúlveda Silva M, Coccio N, Cortés P, et al. Ejercicio intervalado de alta intensidad como terapia para disminuir los factores de riesgo cardiovascular en personas con síndrome metabólico. Nutr Hosp [Internet]. 2015 [citado 21 Feb 2019];132(6):[aprox. 2 p.]. Disponible en: http://scielo.isciii.es/scielo.php?pid=S0212-16112015001200014&script=sci_abstract&tlng=en
2. Ruano Nieto CI, Melo Pérez JD, Mogrovejo Freire L, De Paula Morales KR, Espinoza Romero CV. Prevalencia de síndrome metabólico y factores de riesgo asociados en jóvenes universitarios ecuatorianos. Nutr Hosp [Internet]. 2015 [citado 21 Feb 2019];31(4):[aprox. 2 p.]. Disponible en: http://scielo.isciii.es/pdf/nh/v31n4/16originalsindromemetabolico03.pdf
3. Lopez Siguero JP. Riesgos metabólicos del consumo excesivo de bebidas con azúcares refinados. Nutr Hosp [Internet]. 2016 [citado 21 Feb 2019];33(2):[aprox. 2 p.]. Disponible en: http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S0212-16112016000200001
4. McStea M, McGeechan K, Kamaruzzaman SB, Rajasuriar R, Tan MP. Defining metabolic syndrome and factors associated with metabolic syndrome in a poly-pharmaceutical population. Postgrad Med [Internet]. 2016 [citado 21 Feb 2019]; 128(8):[aprox. 3 p.]. Disponible en: https://www.tandfonline.com/doi/abs/10.1080/00325481.2016.1229103
5. Kaur J. A comprehensive review on metabolic syndrome. Cardiol Res Pract [Internet]. 2014 [citado 21 Feb 2019]:[aprox. 3 p.]. Disponible en: http://downloads.hindawi.com/journals/crp/2014/943162.pdf
6. González Rodríguez R, Cardentey García J. Hábitos de vida saludables en el síndrome metabólico. Rev Ciencias Méd Hab [Internet]. 2016 [citado 21 Feb 2019]; 22(1):[aprox. 3 p.]. Disponible en: http://revcmhabana.sld.cu/index.php/rcmh/article/view/931/134
7. Reaven, GM. Role of Insulin Resistance in Human Disease. Diabetes J [Internet]. 1988 [citado 21 Feb 2019];37(12):[aprox. 2 p.]. Disponible en: http://diabetes.diabetesjournals.org/content/37/12/1595
8. Lam DW, LeRoith D. Metabolic Syndrome. En: De Groot LJ, Chrousos G, Dungan K, Grosmman A, Hershman JM, Kaltsas G, et al. Endotext. 2015 [citado 25 feb 2019]. Disponible en: https://www.ncbi.nlm.nih.gov/books/NBK278936.
9. Fernández Travieso JC. Síndrome Metabólico y Riesgo Cardiovascular. Rev CENIC Cienc Biol [Internet]. 2016 [citado 25 Feb 2019];47(2):[aprox. 15 p.]. Disponible en: http://www.redalyc.org/pdf/1812/181245821006.pdf
10. Castillo JL, Cuevas MJ, Almar M, Romero EY. Síndrome metabólico, un problema de salud pública con diferentes definiciones y criterios. Rev Med UV [Internet]. 2017 [citado 25 Feb 2019];17(2):[aprox. 3 p.]. Disponible en: https://www.medigraphic.com/pdfs/veracruzana/muv-2017/muv172b.pdf
11. Organización Mundial de la Salud. Estadísticas Mundiales. OMS; 2016. Disponible en: www.who.int/gho/publications/world_health_statistics/2016
12. Bustillo E, Pérez Y, Brito A, González A, Montero D, Santos M, et al. Síndrome metabólico un problema de salud no diagnosticado. Rev Cubana Endocrinol [Internet]. 2011 [citado 25 Feb 2019];22(3):[aprox. 2 p.]. Disponible en: http://scielo.sld.cu/scielo.php?pid=S1561-29532011000300001&script=sci_arttext&tlng=en
13. Organización Mundial de la Salud. Enfermedades cardiovasculares. 2015 [citado 20 Mar 2019]. Disponible en: http://www.who.int/mediacentre/factsheats/f5317/es/index.html
14. Pereira Rodríguez JE, Melo Ascanio J, Caballero Chavarro M, Rincón Gonzales G, Jaimes Martin T, Niño Serrato R. Síndrome metabólico. Apuntes de Interés. Rev Cubana Cardiol Cir Cardiovasc [Internet]. 2016 [citado 25 Mar 2019];22(2):[aprox. 9 p.]. Disponible en: http://www.medigraphic.com/pdfs/cubcar/ccc-2016/ccc162i.pdf
15. Ministerio de Salud Pública. Anuario estadístico de salud [Internet]. La Habana: Organización Panamericana de la Salud; 2017 [citado 25 Mar 2019]. Disponible en: https://www.google.com.cu/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=2ahUKEwjE3MnSsYXjAhUvvlkKHSmDCgIQFjAAegQIBRAC&url=http%3A%2F%2Ffiles.sld.cu%2Fdne%2Ffiles%2F2018%2F04%2FAnuario-Electronico-Espa%25C3%25B1ol-2017-ed-2018.pdf&usg=AOvVaw254EWLB-6AynxWrsmiIQQV
16. Moreno MU, Eiros R, Gavira JJ, Gallego C, González A, Ravassa S, et al. The hypertensive myocardium: from microscopic lesions to clinical complications and outcomes. Med Clin North Am [Internet]. 2017 [citado 25 Mar 2019];101(1):[aprox. 2 p.]. Disponible en: https://doi.org/10.1016/j.mcna.2016.08.002
17. Iliev AA, Kotov GN, Dimitrova IN, Landzhov BV. Hypertension-induced changes in the rat myocardium during the development of cardiac hypertrophy – a comparison between the left and the right ventricle. Acta Histochemica [Internet]. 2017 [citado 25 Mar 2019];121:[aprox. 3 p.]. Disponible en: https://doi.org/10.1016/j.acthis.2018.10.004
18. Piskorz D. Corazón en el Síndrome Metabólico. 7mo Curso de Capacitación de Posgrado a Distancia Síndrome Metabólico y Riesgo Vascular; 2013 Ago 2012-2013; Argentina; 2013.
19. Cañón Montañez W, Santos ABS, Foppa M. Strain longitudinal global: un parámetro útil para evaluar disfunción ventricular izquierda subclínica en el síndrome metabólico Rev Colomb Cardiol [Internet]. 2016 [citado 25 Mar 2019];23(2):[aprox. 2 p.]. Disponible en: https://core.ac.uk/download/pdf/82685277.pdf
20. Savova K, Yordanova P, Dimitrov D, Tsenov S, Trendafilov D, Georgieva B. Light microscopic morphological characteristics and data on the ultrastructure of the cardiomyocytes. Acad Anat Int [Internet]. 2017 [citado 25 Mar 2019];3(2):[aprox. 5 p.]. Disponible en: https://aijournals.com/index.php/aanat/article/view/95/91
21. González Madariaga Y, Castillo Alfonso O, Llerena Bernal T, Alfonso Perdomo O, de la Barca Barrera M, González Machado Y. Síndrome metabólico en ratas Wistar inducido por dieta rica en sacarosa. Acta Bioquím Clín Latinoam [Internet]. 2015 [citado 10 Ene 2019];49(3):[aprox. 10 p.]. Disponible en: http://www.redalyc.org/pdf/535/53542622003.pdf
22. Benítez HA, Püschel TA. Modelando la varianza de la forma: Morfometría Geométrica Aplicaciones en Biología Evolutiva. Int J Morphol [Internet]. 2014 [citado 8 Feb 2019];32(3):[aprox. 3 p.]. Disponible en: http://www.scielo.cl/pdf/ijmorphol/v32n3/art41.pdf
23. Iliev AA, Kotov GN, Dimitrova IN, Landzhov BV. Evaluation of structural myocardial changes during chronic hypertensive states in rats. J Cardiol Cardiovasc [Internet]. 2018 [citado 25 Mar 2019];2(1):[aprox. 1 p.]. Disponible en: https://www.researchgate.net/profile/Alexandar_Iliev/publication/322701250_Evaluation_of_structural_myocardial_changes_during_chronic_hypertensive_states_in_rats/links/5a6a1d3f0f7e9b01f3efc87f/Evaluation-of-structural-myocardial-changes-during-chronic-hypertensive-states-in-rats.pdf
24. Castro Barberena A, Martínez Barroso M, Jiménez Estrada G, Martínez Navarro J, Castellanos González M. Cambios morfométricos cardiacos en adultos hipertensos luego de practicar la necropsia. Rev Finlay [Internet]. 2017 [citado 25 Ene 2019];7(2):[aprox. 7 p.]. Disponible en: http://revfinlay.sld.cu/index.php/finlay/article/view/542
25. Leong XF, Ng CY, Jaarin K. Animal models in cardiovascular research: hypertension and atherosclerosis. Biomed Res Int [Internet]. 2015 [citado 6 Mar 2015];[aprox. 5 p.]. Disponible en: http://downloads.hindawi.com/journals/bmri/2015/528757.pdf
26. Dadson K, Kovacevic V, Rengasamy P, et al. Cellular, structural and functional cardiac hypertrophy and healing remodelling following pressure overload and unloading. Int J Cardiol [Internet]. 2016 [citado 6 Mar 2015];216:[aprox. 5 p.]. Disponible en: https://www.google.com.cu/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=2ahUKEwippNGF0IfjAhUCOK0KHdX9C9IQFjAAegQIARAB&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F27140334&usg=AOvVaw2Nyl8_dmDumWB1Z7Atj88q
27. Treskatsch S, Shakibaei M, Feldheiser A, Shaqura M, Dehe L, Roepke TK, et al. Ultrastructural changes associated with myocardial apoptosis, in failing rat hearts induced by volume overload. Int. J. Cardiol [Internet]. 2015 [citado 6 Mar 2015];197(15):[aprox. 3 p.]. Disponible en: https://www.sciencedirect.com/science/article/pii/S016752731530036X
28. Iliev AA, Kotov GN, Landzhov BV. A comparative morphometric study of the myocardium during the postnatal development in normotensive and spontaneously hypertensive rats. Folia Morphol [Internet]. 2018 [citado 6 Mar 2019];77(2):[aprox. 2 p.]. Disponible en: https://journals.viamedica.pl/folia_morphologica/article/download/FM.a2017.0094/44457
29. Barcat JA. Respuestas del corazón: hipertrofia y cicatriz fibrosa. Buenos Aires: Editorial medicina; 2018.
30. Soliman EZ, Prineas RJ. Antihypertensive therapies and left ventricular hypertrophy. Curr Hypertens Rep [Internet]. 2017 [citado 6 Mar 2019];19:[aprox. 2 p.]. Disponible en: https://link.springer.com/article/10.1007/s11906-017-0777-3
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