Hepatic affectations according to morphometry in rats with metabolic syndrome
Keywords:
metabolic syndrome, morphometry, biomodel, disease by non-alcoholic fatty liverAbstract
Introduction: the disease by non-alcoholic fatty liver is considered the hepatic expression of the metabolic syndrome and is accompanied by histological and functional alterations of the liver. The Morphometry is a valuable tool in the assessment of liver morphology.Objective: to describe the histomorphometric differences in the liver of a group of healthy rats and one of them subjected to a state of metabolic syndrome.
Methods: a developmental, correlational, cross-sectional, experimental research study with quantitative approach was conducted in the period from October 2015 to September 2019, using a system of morphometric methods. Histological liver slices were analyzed to 16 male Sprague-Dawley rats randomly distributed in two experimental groups of eight animals for each group. Parametric test (one-factor ANOVA) was applied for group comparisons. The existing relationships between the variables were determined.
Results: the area of the nucleus was greater in the hepatocytes of the perivenous zone in the induced group. The area of the cytoplasm was greater in the hepatocytes of the perivenous zone, but its behavior was homogeneous in the two study groups.
Conclusions: the differences found between the control and induced groups with respect to nuclear and cytoplasmic area could constitute the morphological expression of the biochemical alterations caused by the metabolic syndrome.
Downloads
References
1.Nápoles MB, Pérez Rodríguez OD, Santos MO. Síndrome X. La epidemia del siglo XXI. Rev Infociencia. 2013;17(1):1-12.
2.Rincón Mancheño I. Prevalencia del síndrome metabólico en población española adulta que asiste a consulta dietética [tesis de doctorado]. Madrid: Universidad Complutense de Madrid; 2018 [citado 21/02/2019]. Disponible en: https://eprints.ucm.es/id/eprint/49256/1/T40224.pdf
3.Fall T, Ingelsson E. Genome-wide association studies of obesity and metabolic syndrome. Mol Cell Endocrinol [Internet]. 2014 [citado 21/02/2019];382(1):740-757. Disponible en: https://www.sciencedirect.com/science/article/pii/S0303720712004133. https://doi.org/10.1016/j.mce.2012.08.018
4.Mathiew-Quirós Á, Salinas-Martínez AM, Hernández-Herrera RJ, Gallardo-Vela JA. Síndrome metabólico en trabajadores de un hospital de segundo nivel. Rev Med Inst Mex Seguro Soc [Internet]. 2014 [citado 21/02/2019];52(5):580-587. Disponible en: http://www.medigraphic.com/pdfs/imss/im-2014/im145u.pdf
5.Reaven GM. Role of Insulin Resistance in Human Disease. Diabetes [Internet]. 1988 [citado 21/02/2019];37(12):1595-1607. Disponible en: http://diabetes.diabetesjournals.org/content/37/12/1595
6.Pérez Ramos N, Yanes Milián B, Triana de la Paz I, Álvarez Luna Y, Menéndez Hernández EM, Milián Ramírez O. El hepatocito en el síndrome metabolico. Revisión bibliográfica. Morfovirtual 2020. V Congreso virtual de Ciencias Morfológicas [Internet]. La Habana: CENCOMED; 2020 [citado 22/12/2020]. Disponible en: http://www.morfovirtual2020.sld.cu/index.php/morfovirtual/morfovirtual2020/paper/view/110/123
7.Kaur J. A comprehensive review on Metabolic Syndrome. Cardiol Res Pract [Internet]. 2014 [citado 21/02/2019];2014:943162. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966331/. https://dx.doi.org/10.1155/2014/943162
8.Fernández-Travieso JC. Síndrome Metabólico y Riesgo Cardiovascular. Rev CENIC Cienc Biol [Internet]. 2016 [citado 25/02/2019];47(2):106-119. Disponible en: http://www.redalyc.org/pdf/1812/181245821006.pdf
9.Rojas-Martínez R, Aguilar-Salinas CA, Jiménez-Corona A, Gómez-Pérez FJ, Barquera S, Lazcano-Ponce E. Prevalence of obesity and metabolic syndrome components in Mexican adults without type 2 diabetes or hypertension. Salud Pública Méx [Internet]. 2012 [citado 25/02/2019];54(1):7-12. Disponible en: https://www.medigraphic.com/pdfs/salpubmex/sal-2012/sal121b.pdf
10.Vliet-Ostaptchouk JV, Nuotio ML, Slagter SN, Doiron D, Fischer K, Foco L, et al. The prevalence of metabolic syndrome and metabolically healthy obesity in Europe: a collaborative analysis of ten large cohort studies. BMC Endocr Disord [Internet]. 2014 [citado 25/02/2019];14:9. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923238/. https://dx.doi.org/10.1186/1472-6823-14-9
11.Urióstegui-Flores A, García-Bravo ML, Pérez-Pinto A, Orea-Lara A. Medición de parámetros asociados al síndrome metabólico en alumnos de enfermería en Taxco, México. Rev Salud Pública [Internet]. 2018 [citado 25/02/2019];20(3):334-339. Disponible en: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0124-00642018000300334. https://doi.org/10.15446/rsap.v20n3.53837
12.Gómez Torres FD, González Lemoine M, Legrá Sevila M, Pereña Haber L, López Herrera A. Prevalencia del síndrome metabólico en población de 15 a 74 años del municipio Guantánamo. Rev Inf Cient [Internet]. 2018 [citado 25/02/2019];97(5):987-998. Disponible en: http://www.revinfcientifica.sld.cu/index.php/ric/article/view/2147/3911
13.Wong McClure RA, Gregg EW, Barceló A, Kahye L, Abarca Gómez L, Sanabria López L. Prevalence of metabolic syndrome in Central America: a cross-sectional population-based study. Rev Panam Salud Publica [Internet]. 2015 [citado 21/02/2019];38(3):202-8. Disponible en: https://www.scielosp.org/article/rpsp/2015.v38n3/202-208/en/
14.Ministerio de Salud Pública. Dirección de Registros Médicos y Estadísticas de Salud. Anuario Estadístico de Salud 2018 [Internet]. La Habana: MINSAP; 2019 [citado 22/12/2020]. Disponible en: https://files.sld.cu/bvscuba/files/2019/04/Anuario-Electr%C3%B3nico-Espa%C3%B1ol-2018-ed-2019-compressed.pdf
15. Federación Internacional de la Diabetes. Atlas de la DIABETES de la FID. 6 ed [Internet]. Bruselas: FID; 2016 [citado 21/02/2019]. Disponible en: https://www.fundaciondiabetes.org/upload/publicaciones_ficheros/95/IDF_Atlas_2015_SP_WEB_oct2016.pdf
16.Delgado-Cortés HM, García-Juárez FI, García-Juárez I. La enfermedad por hígado graso no alcohólico y el trabajo del internista. Rev Hosp Jua Mex [Internet]. 2018 [citado 25/02/2019];85(2):86-93. Disponible en: https://www.medigraphic.com/pdfs/juarez/ju-2018/ju182e.pdf
17.Bugianesi E, Leone N, Vanni E, Marchesini G, Brunello F, Carucci P, et al. Expanding the natural history of nonalcoholic steatohepatitis: From cryptogenic cirrhosis to hepatocellular carcinoma. Gastroenterology [Internet]. 2002 [citado 25/02/2019];123(1):134-40. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S0016508502000732. https://doi.org/10.1053/gast.2002.34168
18.Cascales Angosto M. Bases celulares y moleculares de la regeneración hepática. Madrid: Instituto de España; 2008.
19.Ross MH, Kaye GI, Pawlina W. Histología. Texto y Atlas Color con Biología Celular y Molecular. 7a ed. Buenos Aires: Editorial Médica Panamericana; 2015.
20.Rosas C, Vásquez B, del Sol M. Descripción histológica e histoquímica del hígado de cobayo (Cavia porcellus). Int J Morphol [Internet]. 2010 [citado 04/09/2019];28(1):151-156. Disponible en: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100021. http://dx.doi.org/10.4067/S0717-95022010000100021
21.Benítez HA, Püschel TA. Modelando la varianza de la forma: Morfometría Geométrica Aplicaciones en Biología Evolutiva. Int J Morphol [Internet]. 2014 [citado 04/09/2019];32(3):998-1008. Disponible en: http://www.scielo.cl/pdf/ijmorphol/v32n3/art41.pdf
22.Sánchez Freire P. Estudio morfométrico del hígado de ratones en el modelo de hepatoprotección con tres plantas medicinales [tesis]. Villa Clara: Universidad de Ciencias Médicas de Villa Clara; 2015.
23.Hernández Medina MB. Estudio morfométrico del hígado de ratas Wistar en un modelo experimental de hiperlipidemia [tesis]. Villa Clara: Universidad de Ciencias Médicas de Villa Clara; 2011.
24.González Madariaga Y, Castillo Alfonso O, Llerena Bernal T, Alfonso Perdomo O, de la Barca Barrera M, González Machado Y. Síndrome metabólico en ratas Wistar inducido por dieta rica en sacarosa. Acta Bioquím Clín Latinoam [Internet]. 2015 [citado 04/09/2019];49(3):301-309. Disponible en: http://www.redalyc.org/pdf/535/53542622003.pdf
25.Sánchez Valenciano D. Análisis del software Image J para el análisis científico de imágenes [tesis]. Madrid: Universidad Politécnica de Madrid; 2014 [citado 04/09/2019]. Disponible en: http://oa.upm.es/33069/
26.Cornejo R, Garrido O, Jaramillo R. Nuclear morphometric of the steatotic hepatocytes and stimulated steatotisc with infrared laser. Int J Morphol [Internet]. 2016 [citado 04/09/2019];34(1):375-379. Disponible en: https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100054
27.Chang E, Kim L, Park SE, Rhee EJ, Lee WY, Oh KW, et al. Ezetimibe improves hepatic steatosis in relation to autophagy in obese and diabetic rats. World J Gastroenterol [Internet]. 2015 [citado 04/09/2019];21(25):7754-7763. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491962/. https://dx.doi.org/10.3748/wjg.v21.i25.7754
28.Cordero Herrera I, Martin MA, Escrivá F, Álvarez C, Goya L, Ramos S. Cocoa-rich diet ameliorates hepatic insulin resistance by modulating insulin signaling and glucose homeostasis in Zucker diabetic fatty rats. J Nutr Biochem [Internet]. 2015 [citado 04/09/2019];26(7):704-712. Disponible en: https://www.sciencedirect.com/science/article/pii/S0955286315000546. https://doi.org/10.1016/j.jnutbio.2015.01.009
29.Neuschwander Tetri BA. Non-alcoholic fatty liver disease. BMC Med [Internet]. 2017 [citado 04/09/2019];15:45. Disponible en: https://bmcmedicine.biomedcentral.com/articles/10.1186/s12916-017-0806-8. https://doi.org/10.1186/s12916-017-0806-8
30.Díaz Fondén J, Pereira Despaigne OL, León Columbié A, Del Valle Díaz S, Hodelín Tablada R. Relación entre los hallazgos ecográficos, laparoscópicos e histológicos en pacientes con esteatosis hepática no alcohólica. MEDISAN [Internet]. 2015 [citado 04/09/2019];19(3):[aprox. 3 p.]. Disponible en: http://scielo.sld.cu/scielo.php?pid=S1029-30192015000300008&script=sci_arttext&tlng=pt
Downloads
Published
How to Cite
Issue
Section
License
Authors who have publications with this journal agree to the following terms:
- Authors will retain their copyright and assign to the journal the right of first publication of their work, which will simultaneously be subject to a Creative Commons License / Attribution-Noncommercial 4.0 International (CC BY-NC 4.0) that allows third parties to share the work as long as its author and first publication in this journal are indicated.
- Authors may adopt other non-exclusive license agreements for distribution of the published version of the work (e.g., depositing it in an institutional repository or publishing it in a monographic volume) as long as the initial publication in this journal is indicated.
- Authors are allowed and encouraged to disseminate their work through the Internet (e.g., in institutional telematic archives or on their web page) before and during the submission process, which can produce interesting exchanges and increase citations of the published work. (See The effect of open access).
15 junio 2025
