Aspects related to sample collection in the detection of SARS-CoV-2 in Villa Clara

Authors

Keywords:

SARS-Cov-2, COVID-19, specimen handling

Abstract

Introduction: based on the report of a cluster of pneumonia cases of unknown etiology on December 31, 2019 in Wuhan, China, the World Health Organization declared the outbreak to be a public health emergency of international concern; COVID-19 became a pandemic. The process of collecting the sample in the detection of SARS-CoV-2 represents one of the initial and highly relevant stages for carrying out an efficient and robust diagnosis.
Objective: to characterize aspects related to the detection of SARS-CoV-2 and the taking of samples in the Province of Villa Clara.
Methods: a descriptive, observational, retrospective investigation was carried out with the objective of characterizing aspects related to the detection of SARS-CoV-2 and the taking of samples in the Province of Villa Clara from the 157 confirmed cases with the disease in the period of study.
Results: more than half of the cases were taken for bilateral nasal exudate, with a mean threshold cycle of 27.9, with a standard deviation of 7.1. 53.3% of the means of transport corresponded to the Sensure Biotech brand; this type of transport medium was the one with the most samples, with a threshold cycle below 20.
Conclusions: the sample with the highest positivity was the bilateral nasal exudate, which coincides with international authors. Of the means of transport evaluated, Sensure Biotech was the one that offered the best results. In the case of asymptomatic patients, the sample that presented the best results was bilateral nasal exudate.

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Author Biographies

Rafael Abreu Duarte, Centro Provincial de Higiene, Epidemiología y Microbiología, Laboratorio de Biología Molecular

Especialista de I Grado en Medicina General Integral. Especialista de I Grado en Microbiología. Profesor Instructor en la Universidad de Ciencias Médicas de Villa Clara.

María de Lourdes Sánchez Álvarez, Centro Provincial de Higiene, Epidemiología y Microbiología, Laboratorio de Biología Molecular

Especialista de I y II Grado en Microbiología. Profesora Auxiliar en la Universidad de Ciencias Médicas de Villa Clara. Investigadora Agregada.

Liena González Lorenzo, Hospital Ginecoobstétrico Universitario "Mariana Grajales"

Especialista de I Grado en Medicina General Integral. Especialista de I Grado en Microbiología.

Norma Rosario Delgado Cura, Centro Provincial de Higiene, Epidemiología y Microbiología, Laboratorio de Biología Molecular

Licenciada en Bioanálisis Clínico

Yoandy Fidel Rivero Pérez, Centro Provincial de Higiene, Epidemiología y Microbiología, Laboratorio de Biología Molecular

Licenciado en Microbiología. Máster en Investigación y Desarrollo de Medicamentos.

Gretza Sánchez Padrón, Dirección Provincial de Salud Pública de Villa Clara

Especialista de II Grado en Higiene y Epidemiología. Profesora Asistente en la Universidad de Ciencias Médicas de Villa Clara. Investigadora Agregada.

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Published

2022-10-04

How to Cite

1.
Abreu Duarte R, Sánchez Álvarez M de L, González Lorenzo L, Delgado Cura NR, Rivero Pérez YF, Sánchez Padrón G. Aspects related to sample collection in the detection of SARS-CoV-2 in Villa Clara. Acta Méd Centro [Internet]. 2022 Oct. 4 [cited 2025 Jul. 5];16(4):738-46. Available from: https://revactamedicacentro.sld.cu/index.php/amc/article/view/1579

Issue

Vol. 16 No. 4 (2022): October-December

Section

Original Articles

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