Evolution and importance of Molecular Microbiology in the Laboratory of the Provincial Center for Hygiene, Epidemiology and Microbiology
Keywords:
molecular biology, diagnostic platformsAbstract
Introduction: the need to have rapid, sensitive and specific laboratory techniques for the detection of pathogens that are difficult to diagnose led to the creation of the Molecular Biology Laboratory at the Provincial Center of Hygiene, Epidemiology and Microbiology of Villa Clara.Objective: to characterize the evolution and importance of Molecular Microbiology in the Laboratory of the Provincial Center of Hygiene, Epidemiology and Microbiology of Villa Clara from June 2018 to December 2023.
Methods: cross-sectional descriptive study carried out at the Villa Clara Molecular Biology Laboratory in the period from June 2018 to December 2023 based on the total samples processed by polymerase chain reaction, several technological platforms and various kits were used. commercial.
Results: In 2018, molecular diagnosis was implemented with two platforms, Rotor Gene-Q® and Gene Xpert® MTB/RIF, and in 2021, four were available, when the SLAN and the COBAS 4800® platform were introduced. The laboratory became relevant due to the arrival of the 2019 new coronavirus pandemic: 511,225 nasopharyngeal exudate samples and 14,568 determinations were processed for the diagnosis of human immunodeficiency viruses, hepatitis C and B, and human papilloma carried out on the COBAS 4800® platform. In 2023, the pathogen detection capacity was strengthened and the community cocirculation of influenza A and B, parainfluenza virus type 4, the OC 43 coronavirus, SARS-CoV-2 and dengue was identified.
Conclusions: the progressive increase in the resolution capacity of the Molecular Biology Laboratory, together with the implementation of new diagnostic platforms, has allowed us to strengthen the detection of pathogens of clinical interest and improve virological sentinel surveillance.
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1.Lage Dávila A, Molina García JR, Bascó Fuentes EL, Morón Rodríguez F, O'Farrill Mons E. La investigación en salud como elemento integrador entre la universidad y los servicios de salud. Educ Med Super [Internet]. 1995 [citado 04/04/2023];9(1):5-6. Disponible en: https://pesquisa.bvsalud.org/portal/resource/pt/lil-627857
2.Angarita Merchán M, Torres Caicedo MI, Díaz Torres AK. Técnicas de Biología Molecular en el desarrollo de la investigación. Revisión de la literatura. Rev Haban Cienc Méd [Internet]. 2017 [citado 04/04/2023];16(5):796-807. Disponible en: https://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1729-519X2017000500012&lng=es
3.Diz Mellado OM. Técnicas de Biología Molecular en el diagnóstico de enfermedades infecciosas. N Punto [Internet]. 2020 [citado 04/04/2023];3(30):88-111. Disponible en: https://www.npunto.es/revista/30/tecnicas-de-biologia-molecular-en-el-diagnostico-de-enfermedades-infecciosas
4.Govin-Sanjudo A, Pacheco-Cruz Y, Castellanos-Pérez D, Milián-Díaz LC, Herrera-Ledesma Y, Burguet-Lago N, et al. Diagnóstico molecular del SARS-CoV-2 en los Laboratorios LIORAD-AICA+: experiencia de un año de trabajo. Rev Cubana Med Trop [Internet]. 2022 [citado 04/01/2024];74(2):e809. Disponible en: https://revmedtropical.sld.cu/index.php/medtropical/article/view/809
5.Rodríguez-Acosta M, Dubed-Echevarría M, Pérez-Guevara MT, Cruz-Sui O, González-Sosa NL, Hernández-Sánchez T, et al. Experiencia organizativa del Centro de Investigaciones Científicas de la Defensa Civil en el diagnóstico de la COVID-19. Rev Cub Med Mil [Internet]. 2021 [citado 04/01/2024];50(2):e02101326. Disponible en: https://revmedmilitar.sld.cu/index.php/mil/article/view/1326
6.Prensa Latina [Internet]. La Habana: Prensa Latina; 2023 [actualizado 16/04/2021; citado 04/04/2023]. Cuba contará con 27 Laboratorios de Biología Molecular; [aprox. 2 pantallas]. Disponible en: https://www.prensa-latina.cu/2021/04/16/cuba-contara-con-27-laboratorios-de-biologia-molecular
7.Ortiz Jiménez J, Franco Sotomayor G, Ramos Ramírez M. Validación e implementación de GeneXpert MTB/RIF para diagnóstico de tuberculosis en Ecuador. Kasmera [Internet]. 2019 [citado 04/04/2023];47(1):29-37. Disponible en: https://www.redalyc.org/journal/3730/373061540006/html/
8.Amaya G, Contrera M, Arrieta F, Montano A, Pírez C. Rendimiento del GeneXpert en el diagnóstico de tuberculosis pulmonar y extrapulmonar en la edad pediátrica. Arch Pediatr Urug [Internet]. 2020 [citado 05/01/2024];91(Supl 2):12-23. Disponible en: https://www.scielo.edu.uy/scielo.php?script=sci_arttext&pid=S1688-12492020000800012&lng=es. https://doi.org/10.31134/ap.91.s2.2
9.Lacayo de Santana AC, Rodríguez Cruz PG, Pérez Aguilar ZO, Vásquez Cornejo C. Validez diagnóstica del GeneXpert para Mycobacterium tuberculosis y prueba de resistencia a rifampicina. Alerta [Internet]. 2021 [citado 05/01/2024];4(3):176-180. Disponible en: https://docs.bvsalud.org/biblioref/2021/08/1283009/validez-diagnostica.pdf. https://doi.org/10.5377/alerta.v4i3.8829
10.Acosta-Sánchez DR, Domínguez-Sánchez L, López-González J, Duarte-Grandales S. GeneXpert como método de diagnóstico de la tuberculosis en Santiago de Cuba. MEDISAN [Internet]. 2022 [citado 05/01/2024];26(2):255. Disponible en: https://medisan.sld.cu/index.php/san/article/view/3406
11.Mederos Cuervo LM, Martínez Romero MR, Sardiñas Aragón M, García León G, Pereira Gross EG, Díaz Rodríguez R. Aplicabilidad de la herramienta molecular GeneXpert MTB/RIF en el diagnóstico de la Tuberculosis. Rev CENIC Cienc Biol [Internet]. 2020 [citado 05/01/2024];51(3):173-180. Disponible en: https://www.redalyc.org/articulo.oa?id=181272274002
12.Cadavid C, Realpe T, Mejía GI, Zapata E, Hernández M, Robledo J. Contribución del uso de XPERT MTB/RIF y su costo-efectividad en el diagnóstico de tuberculosis pulmonar y la resistencia a rifampicina: una comparación con métodos diagnósticos no moleculares. Infect [Internet]. 2022 [citado 05/01/2024];26(2):121-127. Disponible en: http://revistainfectio.org/P_OJS/index.php/infectio/article/view/1010. https://doi.org/10.22354/in.v26i2.1010
13.Medina-Batalla AL, Kim-Morales D, Abrego-Fernández JA, Laniado-Laborín R. Xpert®MTB/RIF para el diagnóstico de tuberculosis en condiciones programáticas en una región de alta endemicidad en México. Neumol Cir Torax [Internet]. 2019 [citado 05/01/2024];78(2):122-125. Disponible en: https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0028-37462019000200122
14.Manrique-Hinojosa J, Sarria-Bardales G, Núñez-Teran MC, Arias A, Mora P, Sullcahuaman-Allende Y, et al. Detección del virus de papiloma humano por medio del método de cobas® 4800 en mujeres de Lima, Perú. Gac Mex Oncol [Internet]. 2021 [citado 06/01/2024];20(2):46-51. Disponible en: https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2565-005X2021000200046&lng=es. https://doi.org/10.24875/j.gamo.21000093
15.Liu SS, Chan KKL, Wei TN, Tse KY, Ngu SF, Chu MMY, et al. Clinical performance of the Roche Cobas 4800 HPV test for primary cervical cancer screening in a Chinese population. PLoS One [Internet]. 2022 [citado 06/01/2024];17(8):e0272721. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355206/. https://doi.org/10.1371/journal.pone.0272721
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15 junio 2025
